Our nanoparticle technology is the most versatile in the market Reliable and efficient Our nanoparticle emulsions are a patented product, with a stable and controlled production We offer plenty of benefits to your molecule with the highest quality of emulsions. From hydrophobic to amphiphilic molecules, small or complex, we stabilise them and carry them to their target site. Biocompatible With different cell types and in vivo models like mice, rat and zebrafish Stable In different biological fluids, sera and cell culture media High loading capacity Ensuring the desired therapeutic effect Efficient intracellular delivery Overcoming biological barriers Versatile Able to carry a wide range of molecules Nanoemulsion production Assays In vitro assays - Labeling of live cells - Tracking cell internalization - FRET studies - Intracellular delivery of therapeutic compounds - Therapeutic evaluation - Cytotoxicity studies In vivo assays - Biodistribution evaluation - Therapeutic evaluation - Cell tracking Drug delivery of Small molecules Proteins Peptides Nucleic acids Technical data Our proprietary technology is based on lipidic emulsions with an easily controllable size and a narrow distribution. Our nanometric emulsions show great stability in different relevant biological fluids and biocompatibility with different cell types as well as in vivo models such as mouse, rat and zebrafish. They have high loading capacity and are versatile and capable of associating a wide variety of compounds as hydrophobic or amphiphilic small molecules, peptides, proteins, and oligonucleotides (such as DNA and RNA) showing great association efficacy and deliver them to their target site by overcoming biological barriers. DIVERSA’s technology can efficiently achieve the intracellular delivery of therapeutic molecules. Characterization Characterization of size and surface charge expressed as zeta potential, measured by Dynamic Light Scattering (DLS) and ALD. Morphology Morphology of DIVERSA ’s technology observed by Scanning Transmission Electron Microscopy (STEM) and Cryogenic Transmission Electron Microscopy, Cryo-TEM. Internalization Cell internalization in fibroblast of DIVTECH labeled with FluoGreen observed under confocal microscopy after 4 hour incubation. Cell internalization in H1650 spheroids of DIVTECH labeled in red observed under confocal microscopy after 6 hour incubation. Blue: nuclei, red: fibronectin, green: DIVTECH. Blue: nuclei, red: DIVTECH-Cy5, green: cell membranes. Stability Stability of DIVERSA’s technology in different biological relevant fluids. Human plasma Simulated tear fluid Simulated synovial fluid Simulated gastric fluid Simulated intestinal fluid (SIF) Fed state SIF Fasted state SIF DIVTECH 24 h* 24 h* 24 h* 6 h 4 h 4 h 24 h* *End of the experiment, stable particles. Long-term stability at room temperature (40 ºC, 75% RH) and under storage conditions (25 ºC, 60% RH) up to 6 months, and in different buffers and in culture media supplemented with FBS. Biocompatibility DIVERSA ’s technology showed a great biocompatibility with different cell types and increasing concentrations of the nanosystems measured by Alamar assay. Evolution of body weight in mice injected with DIVTECH. Red arrow: one dose tail intravenous injection of 30 mg/kg (10 mg/mL DIVTECH concentration). Gray arrows: three consecutive dose tail intravenous injection of 60 mg/kg (20 mg/mL nanosystem concentration) at days 2, 4 and 6 (cumulative dose 180 mg/kg). Versatility DIVERSA ’s technology can associate a wide variety of compounds as hydrophobic or amphiphilic molecules, peptides, proteins, and oligonucleotides (such as DNA and RNA, among others) showing great association efficiencies. Molecules Association efficiency Small molecules 85-99 % Peptides 70-99 % Proteins 70-99 % DNA/RNA 85-99 %
