NanoGetic team is composed by scientists with recognized experience in the generation of camelid single-domain antibody (sdAb) phage display libraries. Camelids produce a fraction of a unique G-like immunoglobulin isotype that is naturally devoid of the Light chains, named Heavy-chain antibodies (HcAb). The variable domains (VHH) of these HcAbs can be obtained, through recombinant gene technology, as sdAbs. These recombinant sdAbs are the smallest available intact antigen-binding fragment (12-15 kDa) harboring the full antigen recognition capacity. Their exceptional physicochemical properties, possibility of humanization and unique antigen recognition properties make them excellent candidates for targeted delivery of biologically active components. – Immune libraries. NanoGetic S.L. construct these libraries by cloning the VHH genes repertoire, produced by peripheral blood lymphocytes of immunized dromedary with the antigen of interest, in a property phage-display plasmid to finally select antigen-specific VHH fragments (sdAbs). – Non-immune (naïve) libraries. NanoGetic own a larger dromedary sdAbs phage display library to select naturally occur specific sdAbs. – Humanization. sdAbs can be humanized by changing the amino acids 49 (E–G) and 50 (R/C–L) in the framework-2 maintaining their properties and specificity. – sdAbs coupling to nanodevices.
