The ThermoFisher Spectra 300 S/TEM (60–300 keV) is a high-resolution aberration corrected scanning transmission electron microscope. The microscope is equipped with a high-brightness X-FEG source, Cs CETCOR image and S-CORR probe corrector system, Lorentz lens, Super-X EDS detector, DualEELS, and 4D STEM detectors. A suite of sample holders offers a wide range of in situ and operando TEM capabilities. High brightness Schottky field emission gun (X-FEG) with monochromator unit Acceleration voltage: 60 keV, 80 keV, 200 keV and 300 keV Cs CETCOR / S-CORR double aberration corrector system High angle annular dark field (HAADF) STEM detector Panther segmented and on-axis disk and annular STEM detector system EMPAD (Electron Microscope Pixelated Array Detector) for 4D STEM at up to 1100 fps Ceta-S bottom mount TEM camera with speed enhancement Super-X energy dispersive X-ray spectroscopy (EDS) detector GATAN GIF Continuum K3 for EELS, EFTEM, and energy-filtered 4D STEM Cs corrected TEM Lorentz mode (300 keV) for imaging of magnetic domains Precession electron diffraction Energy spread: 0.2–0.3 eV (300 keV) Information limit: 60 pm (300 keV) STEM resolution: 50 pm (300 keV) High-visibility low-background double-tilt holder Low-background single-tilt holder GATAN Model 636 liquid nitrogen cooled double-tilt holder GATAN ELSA 698 cryo-transfer holder with extended tilting range ±70° Analytical tomography holder (high-visibility, low-background, optimized for EDS), ±70° FEI NanoEx–i/v single-tilt TEM holder for MEMS-based heating and biasing Hummingbird Scientific 1300-HF1A Atmospheric Gas TEM holder with 4 electrical contacts Hummingbird Scientific 1400-HF1A Liquid Flow TEM holder with 4 electrical contacts EPU software package for single-particle image acquisition Maps 3 correlative workflow image acquisition and processing software VeloxTM for imaging, EDS and EELS acquisition Gatan Microscopy Suite for experimental control, imaging and spectroscopy applications Inspect3D for tomography data processing and reconstruction Avizo for EM Systems for advanced visualization and analysis of reconstructed tomography data TEM scripting interface for user-defined automation of acquisition workflows High-resolution HAADF- / LAADF- / ABF- / BF-STEM imaging at sub-atomic level High-resolution iDPC imaging for light elements High-resolution TEM imaging Fast EDS elemental mapping with Super-X detector EELS mapping, EFTEM and energy-filtered 4D STEM with Gatan GIF K3 Continuum Monochromated EELS at high energy resolution 4D STEM data acquisition for mapping of strain, phases, orientations, fields, and for DPC and ptychography TEM-, STEM-, and EDS tomography Lorentz microscopy for magnetic and polarized materials in situ and operando TEM capabilities for liquid- and gas-phase, heating, biasing, and cryo studies
