Welcome to the forefront of nanoparticle research with Nanoparticle Tracking Analysis (NTA). This leading-edge technology delivers unparalleled precision, flexibility, and actionable insights for science and industries such as pharmaceuticals, biotechnology, environmental science and materials research. At Particle Metrix we combine decades of expertise with German-engineered systems to offer cutting-edge solutions in nanoparticle analysis. Nanoparticle Tracking Analysis is an advanced technique for characterizing nanoparticles in suspension, covering a size range of 20 to 2000 nm. Using laser illumination and high-sensitivity CMOS cameras, NTA tracks the Brownian motion of every single nanoparticle in a sample in real time. By applying the Stokes-Einstein equation, the technology translates particle movement into precise size measurements. NTA stands out from traditional methods such as Dynamic Light Scattering (DLS) by offering single-particle resolution, enabling the detection of subpopulations within complex mixtures. The technology behind Nanoparticle Tracking Analysis: Laser Illumination: A focused laser beam illuminates nanoparticles in liquid. Scattered light is captured as high-contrast images for precise tracking. High-Resolution CMOS Cameras: Sensitive CMOS cameras enable real-time visualization of particles as small as 10 nm. Brownian Motion Analysis: Random particle motion is tracked to determine mean square displacement, essential for size calculation. Stokes-Einstein Equation: NTA derives particle size from Brownian motion using the Stokes-Einstein equation, ensuring high precision. Fluorescence Detection: Up to four lasers with optical filters detect fluorescently labeled particles for biospecific tagging. Colocalization Algorithms: Advanced NTA systems overlay fluorescence data to identify multiple biomarkers on the same nanoparticle. Why Nanoparticle Tracking Analysis is superior: Single-Particle Resolution: Provides detailed data on individual particles, not just averages. Multi-Parameter Capabilities: Measures size, concentration, zeta potential, fluorescence, and colocalization in one workflow. Broad Sensitivity Range: Detects particles as small as 10 nm at concentrations as low as 10⁵ particles/mL. Real-Time Visualization: Enhances data accuracy by observing particles in motion. Minimal Sample Requirements: Uses small volumes, reducing costs and waste. Colocalization Analysis: Allows studying multiple co-expressed markers on the same vesicle.
