Achieve 15 nm resolution with Magnify™-SOFI on conventional microscopes. This Magnify™-SOFI image was captured using a Nikon Eclipse Ti2 with a CSU-W1 spinning disk confocal module, Andor v.4.2 Zyla sCMOS camera, and a 40×/1.15 water immersion objective. Resolution ~15 nm. The sample, a fully expanded section of the ependymal cell lining from the adult mouse brain, was stained with NHS-ATTO-488 and rendered in 3D to visualize the intricate structure of ependymal cilia and basal bodies. High-resolution 3D imaging of ependymal cilia and basal bodies is essential for understanding their role in cerebrospinal fluid circulation and brain homeostasis. Magnify™-SOFI allows detailed visualization of these structures without needing a expensive and slow serial block-face scanning electron microscope, making it a powerful and accessible tool for studying ciliary function.
