Revolutionizes neural research with rapid 3D synapse mapping, bypassing traditional 2D layering technique by EM. This Magnify™ image was acquired using a Nikon Eclipse Ti2 epifluorescence microscope equipped with a CSU-W1 spinning disk confocal module, Andor v.4.2 Zyla sCMOS camera, and a 40×/1.2 water immersion objective. The sample, an expanded human tissue section, was stained with synaptophysin (yellow) and PSD95 (red). Magnify™ can image 3D synaptic ultrastructure and protein markers with a fraction of the time and cost, accelerating the mapping of brain connectome. Left panel: 3D mapping using EM requires slicing tissue into tens of thousands of layers for 2D imaging, then compiling them into a 3D composite. Right panel: In contrast, Magnify’s rapid 3D mapping with detailed ultrastructure (the first column) and molecular specificity (the second column) revolutionizes our understanding of synaptic function and neural connectivity. The visualization of interactions, such as synaptophysin(yellow) with PSD95(red), is crucial for uncovering the mechanisms of learning and memory, and identifying disruptions linked to neurological
