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A micro-lab on a chip detects blood type within minutes

Blood transfusion, if performed promptly, is a potentially life-saving intervention for someone losing a lot of blood. However, blood comes in several types, some of which are incompatible with others. Transfusing an incompatible blood type can severely harm a patient. It is, therefore, critical for medical staff to know a patient's blood type before they perform a transfusion.

There are four major blood types--O, A, B, and AB. These types differ based on the presence or absence of structures called A antigens and B antigens on the surfaces of red blood cells. Blood can be further divided into positive and negative types based on the presence or absence of D antigens on red blood cells. Medical professionals usually tell a patient's blood type with tests involving antibodies against the A and B antigens. When antibodies recognize the corresponding antigens, they bind to them, causing the blood cells to clump together and the blood to coagulate. Thus, specific antigen-antibody combinations tell us what the blood type of a blood sample is.

Yet, while the concept sounds straightforward, the equipment and techniques required are often very specialized. Tests, therefore, are non-portable, have high personnel cost, and can take over half an hour to yield results. This can prove problematic in several types of emergency situations.

Aiming to solve these problems, a team of scientists at Japan's Tokyo University of Science, led by Dr Ken Yamamoto and Dr Masahiro Motosuke, has developed a fully automated chip that can quickly and reliably determine a patient's blood type. In the words of Dr Motosuke, he and his colleagues "have developed a compact and rapid blood-typing chip which also dilutes whole blood automatically."

The chip contains a micro-sized "laboratory" with various compartments through which the blood sample travels in sequence and is processed until results are obtained. To start the process, a user simply inserts a small amount of blood, presses a button, and waits for the result. Inside the chip, the blood is first diluted with a saline solution and air bubbles are introduced to promote mixing. The diluted blood is transported to a homogenizer where further mixing, driven by more intensely moving bubbles, yields a uniform solution. Portions of the homogenized blood solution are introduced into four different detector chambers. Two chambers each contain reagents that can detect either A antigens or B antigens. A third chamber contains reagents that detect D antigens and a fourth chamber contains only saline solution, with no reagent, and serves as a negative control chamber in which the user should not observe any results. Antigen-antibody reaction will cause blood to coagulate, and by looking at which chambers have coagulated blood, the user can tell the blood type and whether the blood is positive or negative.

Further, the user does not require specialized optical equipment to read the results. The design of the detector chambers allows the easy identification of coagulated blood with the naked eye. The device is also highly sensitive and can even detect weak coagulation.

During testing, the research team screened blood samples from 10 donors and obtained accurate results for all 10 samples. The time needed to determine a single sample's blood type was only five minutes.

Reflecting on the potential benefits of his team's invention, Dr Motosuke remarks, "The advancement of simple and quick blood test chip technologies will lead to the simplification of medical care in emergency situations and will greatly reduce costs and the necessary labor on parts of medical staff." Given the highly portable nature of the chip, Professor Motosuke also speculates that it could be used during aerial medical transport and in disaster response settings. This is a chip that has the potential to change the way emergency medical support is given.

Fully-automatic blood-typing chip exploiting bubbles for quick dilution and detection

Ken Yamamoto, Ryosuke Sakurai, Masahiro Motosuke

Biomicrofluidics, 2020; 14 (2): 024111

DOI: 10.1063/5.0006264

Contact information:

Masahiro Motosuke

Associate Professor, Department of Mechanical Engineering, TUS

Motlab - Micro/nanoscale thermofluidics research group

Tokyo University of Science (TUS)

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